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Learn about large-capacity centrifuges

2019/7/8 11:01:14      click :
Shanghai Luxiangyi Centrifuge Instrument Co., Ltd. has its own industry advantages, has a wealth of experience in the field of centrifuges, and insight into Large capacity centrifuges, combined with popular centrifuges at home and abroad, focusing on the key technologies of centrifuges from functional structure And its progress, and hope to provide useful selection suggestions through technical summarization and comparison of popular models at home and abroad. The AC frequency conversion speed regulation of the centrifuge will gradually replace the DC speed regulation. The speed adjustment system is the core part of the centrifuge. It consists of three major elements: control, power drive and motor. It mainly controls the speed of the motor.
In the development process of Large capacity centrifuges, DC speed regulation is indispensable. Its main features are good braking, wide speed range, simple structure, low cost, and mature theory and practice. It has been widely used in centrifuges and is still being applied and continuously improved, such as improving the wear resistance of DC motor copper heads and carbon brushes to extend the life of Large capacity centrifuge motors and the replacement cycle of carbon brushes. The main disadvantages are that the rectification waveform is poor, the current ripple is large, and the current interruption is easy when the load is light. To maintain the continuity of the DC motor current, a heavy and smooth inductor is added, which increases the volume and weight of the instrument.
Separation of blood cells using a Large capacity centrifuge
(1) Materials and reagents 1. Anticoagulant
(1) Heparin: Heparin is a sulfate-containing mucopolysaccharide, which is usually used as a sodium or potassium salt, which prevents the conversion of prothrombin to thrombin, thereby inhibiting the formation of fibrin from fibrinogen, thereby preventing blood coagulation. The commonly used heparin solution concentration is 1000 U/ml, and the commercially available heparin is mostly 100 U~126 U/mg.
(2) Ethylenediaminetetraacetic acid (EDTA): is a chelating agent. Prepare a 4% solution with physiological saline for use.
(3) Alkali solution (Alsever solution), the formula is as follows: sodium citrate (Na3C6H5O7·5H2O) 0.80g citric acid 0.0325g glucose 2.05g sodium chloride 0.42gH2O added to 100.00ml mixed and dissolved, 114.3 ° C high pressure Steam sterilization for 10 minutes. Aristotle contains both sodium citrate anticoagulant and cell survival nutrients, so it can be used as both an anticoagulant and a blood cell preservation solution.
  2. The needles are made of different types of needles according to different animals and blood collection sites, and must be sterilized or disinfected before use.
3. Blood collection container syringe or flask, etc.
4. Blood collection animals.
The characteristics of the Large capacity centrifuge can be equipped with a variety of rotors. Usually, the rotor is made under the Large capacity centrifuge cup. Therefore, it is very popular in the research laboratory for a variety of experimental capacity requirements, if only a single rotor is available. The price of the centrifuge is relatively high. Once a multi-capacity rotor is used, the price advantage can be clearly reflected.
The Large capacity centrifuge is a powerful centrifugal force generated by the high-speed rotation of the Large capacity centrifuge rotor, which accelerates the sedimentation speed of the particles in the liquid, and separates the sedimentation coefficient and the buoyant density of the sample. It is mainly extracted from the liquid mixture. The composition, according to the density of each substance, after high-speed rotation, the dense liquid sinks to the bottom layer, and the small density liquid floats on it, thus layering the liquid and extracting the pure substance I need. It is applied In many fields such as medicine and chemical industry, Large capacity centrifuges operate at high speed, so it is not possible to touch the drum with the body to prevent accidents. The mesh size of the filter cloth should be determined according to the size of the solid phase particles of the separated material. Otherwise, the separation effect will be affected. In addition, when the filter cloth is installed, the filter cloth sealing ring should be embedded in the drum sealing groove to prevent the material from running in. To ensure the normal operation of the centrifuge, the rotating parts should be refueled once every 6 months. Check the running lubrication condition of the bearing, whether there is wear phenomenon; whether the components in the Large capacity centrifuge brake device are worn, severely replaced; the bearing cover has no leakage Situation. Machine use, cleaning should be ready, keep the machine clean.