Refrigerated high-speed centrifuges can be used together with different rotors, integrating vacuum, high speed and cooling, and can be used for the rapid separation of a large number of cells, subcellular organelles, bacteria and viruses to complete a variety of nucleic acids, proteins, cells and nanometers The separation of materials can meet the experimental needs of relevant professional laboratory personnel such as animal science, food science and life science in our hospital.
　　The liquid contained in the centrifuge tube can not exceed 2/3 of the total capacity when the high-speed centrifuge is centrifuged. Otherwise, the liquid is easy to overflow. Before and after use, care should be taken to ensure that there is no leakage of liquid residue in the rotor and it should be kept dry. When changing the rotor, care should be taken to secure the rotation axis of the centrifuge and the bayonet of the rotor. After the start of centrifugation, the centrifuge can only leave when the centrifuge speed reaches the set speed. Once the centrifuge has abnormality (such as an unbalanced machine, resulting in obvious vibration or noise), immediately press the STOP button. If necessary, Press the power switch directly to cut off the power, stop the centrifugation, and find out the reason.
　　The interface movement method of the Refrigerated high-speed centrifuge can use single-sector cups. After the biological macromolecule sample solution is filled into the analysis cell using the Schlieren optical system, the biological macromolecules leave the gas and liquid meniscus in the pool under the action of the centrifugal force field. Face to determine its settling velocity. If the sample is a protein solution, it can be configured to a concentration of 1 mg/mL. Before adding to the analysis cell, the sample is dialyzed against the buffer in advance. The other balanced single-sector cup is filled with buffer. The Refrigerated high-speed centrifuge meniscus should be as high as the sample-filling cup. The choice of centrifugation speed should be based on the fact that the target protein molecule can completely settle to the bottom of the tank in 1.5 to 2 hours.
　　Judging from the current situation of the use of Refrigerated high-speed centrifuges in our experimental center, the use of two large-scale instruments and equipment is relatively high. Preparation of animal serums, isolation of soy protein, concentration of animal viruses (Newcastle disease virus, bursal disease virus), and utilization of sucrose density Gradient separation and purification of EV71 virus, centrifugal nanomaterials, etc., involving animal medicine, food processing, biochemistry, life sciences and other disciplines, give full play to the efficiency of the use of centrifuges, to meet the experimental needs of related disciplines.
　　The commonly used Refrigerated high-speed centrifuge tubes used in laboratories are plastic and glass materials, plastics are more common, and the centrifuge tubes of glass materials are fragile and cannot be used in high-speed or Refrigerated high-speed centrifuges. The plastic centrifuge tube is made of polypropylene, polycarbonate, polyethylene, etc. The plastic centrifuge tube is transparent or translucent. The Refrigerated high-speed centrifuge can intuitively see the centrifugal condition of the sample, but it is easily deformed and corrosive against organic solvents. Poor, so the service life is shorter.