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Laboratory centrifuge separation method introduction

2018/5/7 13:53:39      click :
After the name suggests is a laboratory centrifuge microcentrifuge for experimental analysis of the professional, such as to make a mixture of two different molecular weight proteins isolated by two different proteins of mass principle, was transferred at a certain speed, the Centrifuge period You can separate the two proteins. It can be divided into industrial laboratory centrifuges and medical laboratory centrifuges depending on the applications. Industrial laboratory centrifuges are generally used for industrial solid-liquid separation tests, material performance analysis, small-scale separation processes and corporate laboratories. Medical laboratory centrifuges are mainly used in medical experiments, hospitals, pharmaceutical companies, biological institutes, fine chemicals, etc.
The Laboratory centrifuge separation method is different, and the application is not the same. Most centrifugal separation method is the mainstream of differential centrifugation and zonal heart, in fact, there are two types of centrifugation method are mentioned in a centrifuge laboratory ultracentrifugation article technology preparation, Xiao Bian today these two methods and principles Introduction to the application to complement and improve.
1. Differential centrifugation: Many and subcellular biological macromolecules that have a biological activity is unstable, requires centrifugation at low speed, the most commonly used is differential centrifugation. After separating by centrifugation and the supernatant was decanted and the precipitate, the supernatant was separated by speed centrifugation is increased, the precipitate is separated second portion, so that the increase in the rotational speed, the sequential separation of the desired substance . This method can effectively separate particles with large size differences, but can not separate particles of similar size, and separate components are often not uniform, mixed with other types of particles.
2. Centrifuging the zone: It can be divided into two types: centrifugation of the velocity zone and centrifugation of the zone of constant density velocity.
1) Centrifugation of the velocity zone: it involves placing a small amount of suspension in a gradient of soft density and using this gradient to stabilize the sedimentation of the particles. Due to centrifugation, the particles move away from the local area, the speed of movement by the centrifugal force of particle size, shape, and underwent a laboratory centrifuge to decide. After centrifuging for a period of time, the various granules are separated according to their relative velocities and become a series of zones. In this way, we can use particles with a sedimentation velocity difference of 20% or more.
2) Centrifugation of the iso-density zone: This is the suspension of the particles to be separated in the density gradient, or the particles are actually dissolved in a gradient solution. By centrifugation, the particles float or sink to the same density as their own liquid where they have no weight and no matter how long they take to centrifuge, they no longer move. These particles can become a series of zones, each in its own density zone. Therefore, this is a method to separate the particle size using the floating density of particles. The medium used is usually cesium chloride (Cscl) and barium sulfate (Cs2SO4). The former is suitable for DNA isolation and the latter is suitable for RNA isolation. This method, in comparison with the different zonal rate, which has a great disadvantage is that during centrifugation, the particles inevitably exposed to a high concentration gradient of the solution, can cause some damage to the particles, and the particles corresponding to possible Damages Some extra zones
Laboratory centrifuge from the name we can know that it is a dedicated laboratory equipment, which is to use the centrifugal force to separate the solution quickly mixed specialized equipment, such as: biological cells, proteins and other organic solution, inorganic and colloidal samples they separated, they concentrated and they were extracted.