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Precautions when using the centrifuge

2018/9/14 10:23:09      click :
Centrifuges have many advantages as a means. For example, ultracentrifugation can be operated at low temperatures, protecting the activity of biological macromolecules. The preparative centrifuge has a large loading capacity and can separate and purify a few grams of sample at a time, which is much larger than that of chromatography and electrophoresis. The analysis centrifuge can not only measure the molecular weight of the substance, but also the purity, conformation and sedimentation coefficient of the substance. Therefore, centrifugation technology plays an important role in biological research and is a convenient and effective tool for isolating and purifying cells, viruses, proteins, nucleic acids and enzymes.

So what are the precautions when using a centrifuge?

Centrifuge use precautions:
The laboratory is commonly used in electric centrifuges. The electric centrifuge rotates at a high speed. It is necessary to pay attention to safety. In particular, it is necessary to prevent the centrifuge from moving while working during the operation of the centrifuge, so that it moves from the bench. When it falls, or the cover is not covered, the centrifuge tube is broken by vibration, and the glass fragments are rotated and flew out, causing an accident. Therefore, the following operations must be taken into account when using the centrifuge.
(1) The bottom of the centrifuge casing should be padded with cotton or test tube pads.
(2) If the electric centrifuge is vibrating or the body is vibrating, the power should be cut off immediately to eliminate the fault immediately.
(3) The centrifuge tube must be placed symmetrically into the casing to prevent the body from vibrating. If only one sample tube is to be replaced with water of equal quality.
(4) When starting the centrifuge, the top cover of the centrifuge should be covered before it can be started slowly.
(5) After the separation is completed, the centrifuge is turned off first, and after the centrifuge stops rotating, the centrifuge cover can be opened to take out the sample, and no external force can be used to force the movement to stop.
(6) The centrifugation time is generally 1 to 2 minutes. During this period, the experimenter must not leave to do other things.
(7) Note on electric use: In the research institutes of enterprises or institutions, low-speed experimental centrifuges with a rotational speed of about 3000 rpm have been commonly used, such as 315 type filtration and sedimentation integrated experimental centrifuge, 100 type experimental centrifuge, type 200. Experimental centrifuges, etc. These centrifuges are reasonably designed, have a low center of gravity, are safe and stable, and have gained greater application in the analysis of pre-samples in the chemical, food, and medical fields.
The centrifuge is as smooth and smooth as possible in the structural design, and the surface is neat, expelling the corners of the body and repelling dead spots such as effusion and accumulation. All stainless steel surfaces should be polished and polished to a smooth surface. The centrifuge is as smooth and smooth as possible in the structural design, and the surface is neat, expelling the corners of the body and repelling dead spots such as effusion and accumulation. All stainless steel surfaces should be polished and polished to a smooth surface.